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KMID : 0378019740170070941
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New Medical Journal
1974 Volume.17 No. 7 p.941 ~ p.949
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Histocheinical Study on the Effect of Azathioprine and Pilocarpine on the Salivary Gland of the Albino Rat
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Abstract
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Azathioprine is widely used in preventing the rejection of renal homografts and in the treatment of diseases considered to be autoimmune in origin. In view of the recent works, since the 6-mercaptop￢urine moiety released from azathioprine probably interferes with both the de move synthesis of purine and the interconversion of purine nucleotides, potential effects of azathioprine on the synthesis of both DNA and RNA might be expected. In addition to these actions, azathioprine can be converted directly to 6-thiouric acid without the release of 6-mercaptopurine, thereby perhaps allowing a mec￢hanism of action unique to azathioprine.
This study observed the histochemical features produced in the submaxillary and the parotid glands following parenteral administration of a single dose of azathioprine, and azathioprine in combination with pilocarpine.
For this study forty nine male albino rats were used. The average weight of the animals was approximately 100g. These rats were randomly divided into three groups. Forty two rats in the first group(twenty one rats) and the second group(twenty one rats) were intraperitoneally injected with azathioprine at a dose equivalent to 5mg/kg body weight in 0.01 N NaOH solution 1 ml. Ig the above both group, salivary glands were removed on days, 1, 2, 3, 5, 7, 10 and 14 followii injection under ether anesthesia. In the second group of twenty one animals, each was injected if traperitone￢ally with pilocarpine in the amount of 1 mg/kg body weight in distilled water 1 ml two hours prior to sacrifice respectively. Seven aramals of control group were injected with 0. 01 N NaOH solution 1 ml.
All salivary glands were embedded in paraffin by an electric vacuum pump. The histochemical techniques employed can be classified as follows:
(A) Protein: alloxan Schiff ´reaction,´(´Yasma,-rchigawa: ), -Si l´ reaction and-SS group reaction (Pearse )
(B) Glycoprotein: mucicarmine -stain (Soughgate´s modification, of Mayer´s `method).
(C) Glycogen: PAS reaction (McManus), PAS reaction after salivary test (Bensely).
(D) Neutral mucopolysaccharide: acetylation-PAS, acetylation-saponification-PAS, periodic acid￢
paradiamine (Spicer).
(E) Acid mucopolysaccharide: alcian blue 8 GX(pH 2.2), colloidal iron (Muller-Mowry), aldehyde fuchsin(Gomori).
(F) Alkaline phosphatase: diazo reaction(pH 9.2).
(G) Acid phosphatase: diazo reaction (pH 5.0).
(H) DNA and RNA: methyl green-pyronin (Taft).
The results were as follows:
(1) In the azathioprine administered groups, acinar cells had vacuolization from five to ten days after injection.
(2) Azathioprine led in the submaxillary and the parotid gland to a decrease of secretory granule and exclusively of a-amino acid, -SH group and -glycoprotein.
(3) Azathioprine led to a increase of acid phosphatase activity in the acinar cells from five to ten days after injection.
(4) It was seemed that pilocarpine had not acted on the a-amino acid and glycoprotein reaction decreased markedly by azathioprine.
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KEYWORD
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